Production of L-asparaginase by filamentous fungi.
نویسندگان
چکیده
L-asparaginase production was investigated in the filamentous fungi Aspergillus tamarii and Aspergillus terreus. The fungi were cultivated in medium containing different nitrogen sources. A. terreus showed the highest L-asparaginase (activity) production level (58 U/L) when cultivated in a 2% proline medium. Both fungi presented the lowest level of L-asparaginase production in the presence of glutamine and urea as nitrogen sources. These results suggest that L-asparaginase production by of filamentous fungi is under nitrogen regulation.
منابع مشابه
Industrial Production and Clinical Application of L-Asparaginase: A Chemotherapeutic Agent
This article comprises detail information about Lasparaginase, encompassing topic such as various sources of Lasparaginase, mechanism and properties of L-asparaginase. Also describe the production, cultivation and purification of Lasparaginase along with information about the application of Lasparaginase. L-asparaginase catalyzes the conversion reaction to convert asparagine to aspartic acid an...
متن کاملIndustrial Production and Clinical Application of L-Asparaginase: A Chemotherapeutic Agent
This article comprises detail information about Lasparaginase, encompassing topic such as various sources of Lasparaginase, mechanism and properties of L-asparaginase. Also describe the production, cultivation and purification of Lasparaginase along with information about the application of Lasparaginase. L-asparaginase catalyzes the conversion reaction to convert asparagine to aspartic acid an...
متن کاملIsolation and identification of L-asparaginase producing actinomycetes from Persian Gulf
Introduction: L-asparaginase is an anti-neoblastic agent used in the chemotherapy of lymphoblastic leukemia. This enzyme is widely distributed among microorganisms, plants and animals, but microorganisms have proved to be a better alternative for L-asparaginase, thus facilitating its large-scale production. Actinomycetes are filamentous, Gram-positive bacteria widely distributed in the marine e...
متن کاملEvaluation and Screening of Nitrogen Source for L-asparaginase Production by Aspergillus terreus MTCC 1782 using Latin Square Design
The manufacture of enzymes, like L-asparaginase with less adverse effects in the treatment of cancer is an important facet of pharmaceutical industry. Yeast and Filamentous fungi are commonly reported in scientific literature to produce L-asparaginase with less adverse effects than prokaryotic microorganisms. In the present study Latin square design was used to examine the effect of L-proline, ...
متن کاملProduction of L-Asparaginase by Serratia marcescens SB08: Optimization by Response Surface Methodology
This paper describes optimization method that combines the Plackett-Burman design, a factorial design and the response surface method, which were used to optimize the medium for the production of L-asparaginase by Serratia marcescens SB08. Four medium factors, from out of 11 medium factors, were screened by Plackett-Burman design experiments and subsequent optimization process to find out the o...
متن کاملذخیره در منابع من
با ذخیره ی این منبع در منابع من، دسترسی به آن را برای استفاده های بعدی آسان تر کنید
برای دانلود متن کامل این مقاله و بیش از 32 میلیون مقاله دیگر ابتدا ثبت نام کنید
ثبت ناماگر عضو سایت هستید لطفا وارد حساب کاربری خود شوید
ورودعنوان ژورنال:
- Memorias do Instituto Oswaldo Cruz
دوره 99 5 شماره
صفحات -
تاریخ انتشار 2004